human dermal fibroblast nucleofector kit (Lonza)

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Lonza human dermal fibroblast nucleofector kit
Human Dermal Fibroblast Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+dermal+fibroblast+nucleofector+kit/4d+nucleofector/bio_rxiv__2025__06__05__657880-173-7-12
Average 90 stars, based on 1 article reviews

human dermal fibroblast nucleofector kit - by Bioz Stars, 2026-09

90/100 stars

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Knockdown:

Article Title: Dysregulated NUB1 and neddylation enhances rheumatoid arthritis fibroblast-like synoviocyte inflammatory responses.
Article Snippet: NUB1 was overexpressed using 1.5 μg of pRP-CMV-hNUB1[NM_016118.5] or control empty vector (VectorBuilder) for 48 hours. .. For NUB1 knockdown, 1 μg of ON-TARGETplus SMARTpool siRNA targeting human NUB1 (Dharmacon) or ON-TARGET plus Nontargeting Pool siRNA (Dharmacon) were transfected using the Human Dermal Fibroblast Nucleofector Kit (Lonza). .. For luciferase assays, 1ug of pGL4.32[luc2P/NF-κB-RE/Hygro] Vector Protocol (Promega), was co-transfected with 50 ng renilla plasmid into RA FLS.

Transfection:

Article Title: IL-17A and TNF synergistically drive expression of proinflammatory mediators in synovial fibroblasts via IκBζ-dependent induction of ELF3
Article Snippet: .. Synovial fibroblasts were nucleofected using Amaxa Nucleofector II and Human Dermal Fibroblast Nucleofector Kit (Lonza, Basel, Switzerland), using 4 × 10 5 cells, 3 μg DNA and transfection program U023. ..

Article Title: Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.
Article Snippet: .. To generate a virus from the purified viral BAC DNA, HF cells were transfected with BAC DNA (2–3 μL) and a plasmid containing pp65 (0.5 μg) using the Human Dermal Fibroblast Nucleofector Kit (Lonza, #VPD-1001) as described previously. ..

Article Title: Loss of alcohol dehydrogenase 1B in cancer-associated fibroblasts: contribution to the increase of tumor-promoting IL-6 in colon cancer.
Article Snippet: BACKGROUND: Increases in IL-6 by cancer-associated fibroblasts (CAFs) contribute to colon cancer progression, but the mechanisms involved in the increase of this tumor-promoting cytokine are unknown.. The aim of this study was to identify novel targets involved in the dysregulation of IL-6 expression by CAFs in colon cancer.. METHODS: Colonic normal (N), hyperplastic, tubular adenoma, adenocarcinoma tissues, and tissue-derived myo-/fibroblasts (MFs) were used in these studies.

Article Title: The HRAS-binding C2 domain of PLCη2 suppresses tumor-like synoviocytes and experimental arthritis in rheumatoid arthritis.
Article Snippet: .. The DNA and shRNA vectors were transfected separately into cells using the Human Dermal Fibroblast Nucleofector Kit (Lonza) according to the manufacturer’s protocol. .. In brief, 5 × 105 cells were resuspended in 100 μl of nucleofector solution containing DNA or shRNA plasmids and electroporated with the U-23 program on an Amaxa Nucleofector II device.

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Article Title: iPSC-derived skeletal muscle spheroids for Duchenne Muscular Dystrophy modeling
Article Snippet: Fibroblasts or PBMCs were reprogrammed using the Human Dermal Fibroblast NucleofectorTM Kit (Lonza) or Human CD34 + Cell Nucleofector TM Kit (Lonza), according to the manufacturer’s instructions, with the episomal vectors pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, and pCXLE-hUL (Addgene) using NucleofectorTM 2b Device.

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